NTC
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SKPA
Gene Ontology Biological Process
- DNA endoreduplication [IMP]
- centrosome duplication [IMP]
- chromosome condensation [IMP]
- mitotic G2 DNA damage checkpoint [IGI]
- mitotic nuclear division [IMP]
- negative regulation of JNK cascade [IMP]
- negative regulation of apoptotic process [IGI, IMP]
- negative regulation of insulin receptor signaling pathway [IGI]
- negative regulation of synaptic growth at neuromuscular junction [IMP]
- neurogenesis [IMP]
- neuron remodeling [IMP]
- positive regulation of mitotic cell cycle [IMP]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [ISS]
- regulation of centrosome duplication [IMP]
- regulation of protein stability [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
A novel F-box protein is required for caspase activation during cellular remodeling in Drosophila.
Terminal differentiation of male germ cells in Drosophila and mammals requires extensive cytoarchitectural remodeling, the elimination of many organelles, and a large reduction in cell volume. The associated process, termed spermatid individualization, is facilitated by the apoptotic machinery, including caspases, but does not result in cell death. From a screen for genes defective in caspase activation in this system, we ... [more]
Throughput
- Low Throughput