CG3680
Gene Ontology Molecular Function
SU(VAR)205
Gene Ontology Biological Process
- DNA hypermethylation [IMP]
- chromatin silencing [IMP]
- chromatin silencing at centromere [IMP]
- chromosome organization [IMP]
- establishment of chromatin silencing [IMP]
- gene silencing [IDA, IMP]
- heterochromatin assembly [IDA]
- histone H4-K20 trimethylation [IMP]
- negative regulation of transcription, DNA-templated [NAS]
- neurogenesis [IMP]
- positive regulation of extent of heterochromatin assembly [IMP]
- positive regulation of histone H3-K9 dimethylation [IDA, IMP]
- positive regulation of transcription, DNA-templated [TAS]
- regulation of apoptotic process [IMP]
- regulation of histone methylation [IMP]
- regulation of transcription, DNA-templated [IMP]
- telomere maintenance [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- chromosome [IDA]
- chromosome, centromeric region [NAS, TAS]
- chromosome, telomeric region [IDA]
- condensed chromosome [IDA]
- condensed nuclear chromosome, centromeric region [IDA]
- euchromatin [IDA]
- heterochromatin [IDA, NAS, TAS]
- nuclear heterochromatin [IDA]
- nucleus [IDA, NAS]
- pericentric heterochromatin [IDA]
- polytene chromosome [IDA]
- polytene chromosome band [IDA]
- polytene chromosome chromocenter [IDA, TAS]
- polytene chromosome puff [IDA, TAS]
- polytene chromosome, telomeric region [IDA, TAS]
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Heterochromatin-associated interactions of Drosophila HP1a with dADD1, HIPP1, and repetitive RNAs.
Heterochromatin protein 1 (HP1a) has conserved roles in gene silencing and heterochromatin and is also implicated in transcription, DNA replication, and repair. Here we identify chromatin-associated protein and RNA interactions of HP1a by BioTAP-XL mass spectrometry and sequencing from Drosophila S2 cells, embryos, larvae, and adults. Our results reveal an extensive list of known and novel HP1a-interacting proteins, of which ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SU(VAR)205 CG3680 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | FlyBase | - | |
| CG3680 SU(VAR)205 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
| CG3680 SU(VAR)205 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |