TFIIA-S
Gene Ontology Biological Process
Gene Ontology Molecular Function
TFIIA-L
Gene Ontology Biological Process
Gene Ontology Molecular Function- RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly [IPI]
- TBP-class protein binding [IPI]
- TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IPI]
- TFIID-class transcription factor binding [IPI]
- RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly [IPI]
- TBP-class protein binding [IPI]
- TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IPI]
- TFIID-class transcription factor binding [IPI]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Drosophila TFIIA directs cooperative DNA binding with TBP and mediates transcriptional activation.
Drosophila transcription factor IIA (TFIIA) is composed of three subunits (30, 20, and 14 kD) that function during initiation of transcription. We reported previously the characterization of cDNAs that encode a precursor (dTFIIA-L) of the Drosophila TFIIA 30- and 20-kD subunits. In the absence of the smallest subunit, dTFIIA-S (14 kD), the unprocessed large subunit failed to exhibit any detectable ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
TFIIA-L TFIIA-S | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
TFIIA-S TFIIA-L | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |