CG18112, Dmel\CG18112, dVps16B, fob, Dmel_CG18112
Vacuolar protein sorting 16B
GO Process (5)
GO Function (1)
GO Component (3)
Drosophila melanogaster


CG5127, Dmel\CG5127, Vps 33B, dVps33B, Dmel_CG5127
Vacuolar protein sorting 33B
Drosophila melanogaster

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.


The full-of-bacteria gene is required for phagosome maturation during immune defense in Drosophila.

Akbar MA, Tracy C, Kahr WH, Kraemer H

Arthrogryposis, renal dysfunction, and cholestasis (ARC) syndrome is a fatal recessive disorder caused by mutations in the VPS33B or VPS16B genes. Both encode homologues of the Vps33p and Vps16p subunits of the HOPS complex necessary for fusions of vacuoles in yeast. Here, we describe a mutation in the full-of-bacteria (fob) gene, which encodes Drosophila Vps16B. Flies null for fob are ... [more]

J. Cell Biol. Feb. 07, 2011; 192(3);383-90 [Pubmed: 21282466]


  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.


Curated By