BAIT

FOR

142251_at, BcDNA:GM08338, CG10033, DG2, Dmel\CG10033, FOR/PKG, PKG, Pkg2, Pkg24A, anon-WO0140519.260, anon-WO02059370.47, l(2)06860, Dmel_CG10033
foraging
Drosophila melanogaster
PREY

LOLA

BEST:LD03274, BTB-IV, BcDNA:LD17006, BcDNA:RH31485, BtbIV, CG12052, CG18376, CG18378, CG18379, CG18380, CG18381, CG30012, CG30013, CG30014, Dmel\CG12052, LD03274, NEST:bs06a08, bs06a08.y1, eyeful, fus7, l(2)00642, l(2)s3697, sw59, Dmel_CG12052
longitudinals lacking
Drosophila melanogaster

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

cGMP-Dependent Protein Kinase Encoded by foraging Regulates Motor Axon Guidance in Drosophila by Suppressing Lola Function.

Peng Q, Wang Y, Li M, Yuan D, Xu M, Li C, Gong Z, Jiao R, Liu L

Correct pathfinding and target recognition of a developing axon are exquisitely regulated processes that require multiple guidance factors. Among these factors, the second messengers, cAMP and cGMP, are known to be involved in establishing the guidance cues for axon growth through different intracellular signaling pathways. However, whether and how cGMP-dependent protein kinase (PKG) regulates axon guidance remains poorly understood. Here, ... [more]

J. Neurosci. Apr. 20, 2016; 36(16);4635-46 [Pubmed: 27098704]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
LOLA FOR
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-FlyBase
-
LOLA FOR
Phenotypic Suppression
Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-FlyBase
-
FOR LOLA
Phenotypic Suppression
Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-FlyBase
-
FOR LOLA
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By