BAIT
BRC1
SPBC582.05c
BRCT domain protein Brc1
GO Process (2)
GO Function (1)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Schizosaccharomyces pombe (972h)
PREY
SWI1
SPBC216.06c
replication fork protection complex subunit Swi1
GO Process (6)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Schizosaccharomyces pombe (972h)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Ku stabilizes replication forks in the absence of Brc1.
DNA replication errors are a major source of genome instability in all organisms. In the fission yeast Schizosaccharomyces pombe, the DNA damage response protein Brc1 binds phospho-histone H2A (γH2A)-marked chromatin during S-phase, but how Brc1 protects genome integrity remains unclear. Here we report that the non-homologous end-joining (NHEJ) protein Ku becomes critical for survival of replication stress in brc1∆ cells. ... [more]
PLoS ONE May. 13, 2015; 10(5);e0126598 [Pubmed: 25965521]
Throughput
- Low Throughput
Ontology Terms
- phenotype: viability (APO:0000111)
Additional Notes
- cis-phenotypic enhancing
- double mutants show increased sensitivity to DNA damaging agents
Curated By
- BioGRID