A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Characterization of a Novel MMS-Sensitive Allele of Schizosaccharomyces pombe mcm4.

Ranatunga NS, Forsburg SL

The minichromosome maintenance (MCM) complex is the conserved helicase motor of the eukaryotic replication fork. Mutations in the Mcm4 subunit are associated with replication stress and double strand breaks in multiple systems. In this work we characterize a new temperature sensitive allele of Schizosaccharomyces pombe mcm4(+) Uniquely amongst known mcm4 alleles, this mutation causes sensitivity to the alkylation damaging agent ... [more]

G3 (Bethesda) Jul. 29, 2016; 0(0); [Pubmed: 27473316]

Throughput

Low Throughput

Ontology Terms

phenotype: viability (APO:0000111)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
HSK1 MCM4	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Takeda T (2001)	Low	-	BioGRID	247599

Curated By

BioGRID

Interaction Summary

MCM4

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP-dependent 3'-5' DNA helicase activity [IDA]ATPase activity [IDA]chromatin binding [IDA]single-stranded DNA binding [IDA]single-stranded DNA-dependent ATP-dependent DNA helicase activity [IDA]

Gene Ontology Cellular Component

HSK1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [IDA]protein binding [IPI]protein kinase activity [IDA]protein serine/threonine kinase activity [IDA]