Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Synaptic GAP and GEF Complexes Cluster Proteins Essential for GTP Signaling.

Wilkinson B, Li J, Coba MP

GTPase-activating proteins (GAPs) and guanine exchange factors (GEFs) play essential roles in regulating the activity of small GTPases. Several GAPs and GEFs have been shown to be present at the postsynaptic density (PSD) within excitatory glutamatergic neurons and regulate the activity of glutamate receptors. However, it is not known how synaptic GAP and GEF proteins are organized within the PSD ... [more]

Sci Rep Jul. 13, 2017; 7(1);5272 [Pubmed: 28706196]

Throughput

  • High Throughput

Additional Notes

  • Non-postsynaptic density (PSD) interactions

Curated By

  • BioGRID