BAIT

ABO1

SPAC31G5.19, SPAC31G5.19
ATPase with bromodomain protein (predicted)
GO Process (2)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Schizosaccharomyces pombe (972h)
PREY

HHT2

h3.2, SPBC8D2.04
histone H3 h3.2
GO Process (2)
GO Function (2)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Schizosaccharomyces pombe (972h)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Abo1, a conserved bromodomain AAA-ATPase, maintains global nucleosome occupancy and organisation.

Gal C, Murton HE, Subramanian L, Whale AJ, Moore KM, Paszkiewicz K, Codlin S, Baehler J, Creamer KM, Partridge JF, Allshire RC, Kent NA, Whitehall SK

Maintenance of the correct level and organisation of nucleosomes is crucial for genome function. Here, we uncover a role for a conserved bromodomain AAA-ATPase, Abo1, in the maintenance of nucleosome architecture in fission yeast. Cells lacking abo1(+) experience both a reduction and mis-positioning of nucleosomes at transcribed sequences in addition to increased intragenic transcription, phenotypes that are hallmarks of defective ... [more]

EMBO Rep. Jan. 01, 2016; 17(1);79-93 [Pubmed: 26582768]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: viability (APO:0000111)
  • phenotype: resistance to chemicals (APO:0000087)
  • phenotype: heat sensitivity (APO:0000147)

Additional Notes

  • deletion of abo1 in the non-lethal hht2/hhf2 mutant causes cell death at high temperatures and in the presence of MMS
  • genetic complex

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ABO1 HHT2
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID