DLG4
Gene Ontology Biological Process
- alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor clustering [IBA, ISO]
- dendritic spine morphogenesis [IDA, ISO]
- establishment of protein localization [ISO]
- locomotory behavior [NAS]
- locomotory exploration behavior [IMP]
- negative regulation of receptor internalization [ISO]
- neuromuscular process controlling balance [IMP]
- nucleotide phosphorylation [IBA]
- positive regulation of cytosolic calcium ion concentration [ISO]
- positive regulation of excitatory postsynaptic membrane potential [ISO]
- positive regulation of synaptic transmission [ISO]
- protein complex assembly [ISO]
- protein localization to synapse [IDA, ISO]
- receptor localization to synapse [IBA, ISO]
- regulation of N-methyl-D-aspartate selective glutamate receptor activity [ISO]
- regulation of grooming behavior [IMP]
- regulation of long-term neuronal synaptic plasticity [IGI]
- regulation of neuronal synaptic plasticity [NAS]
- response to cocaine [NAS]
- signal transduction [IBA]
- social behavior [IMP]
- synaptic vesicle maturation [IDA, IGI]
- vocalization behavior [IMP]
Gene Ontology Molecular Function- D1 dopamine receptor binding [ISO]
- P2Y1 nucleotide receptor binding [ISO]
- PDZ domain binding [ISO]
- acetylcholine receptor binding [ISO]
- beta-1 adrenergic receptor binding [ISO]
- glutamate receptor binding [ISO]
- guanylate kinase activity [IBA]
- ionotropic glutamate receptor binding [IPI, ISO]
- kinase binding [ISO]
- neurexin family protein binding [NAS]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein kinase binding [ISO]
- protein phosphatase binding [ISO]
- receptor binding [ISO]
- scaffold protein binding [IPI]
- structural molecule activity [NAS]
- D1 dopamine receptor binding [ISO]
- P2Y1 nucleotide receptor binding [ISO]
- PDZ domain binding [ISO]
- acetylcholine receptor binding [ISO]
- beta-1 adrenergic receptor binding [ISO]
- glutamate receptor binding [ISO]
- guanylate kinase activity [IBA]
- ionotropic glutamate receptor binding [IPI, ISO]
- kinase binding [ISO]
- neurexin family protein binding [NAS]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein kinase binding [ISO]
- protein phosphatase binding [ISO]
- receptor binding [ISO]
- scaffold protein binding [IPI]
- structural molecule activity [NAS]
Gene Ontology Cellular Component
- alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid selective glutamate receptor complex [IDA]
- cell junction [IDA]
- cerebellar mossy fiber [IDA]
- cortical cytoskeleton [ISO]
- cytoplasm [IDA]
- cytosol [ISO]
- dendrite [IDA]
- dendrite cytoplasm [ISO]
- endoplasmic reticulum [IDA]
- excitatory synapse [IDA, ISO]
- extrinsic component of cytoplasmic side of plasma membrane [IDA]
- ionotropic glutamate receptor complex [IDA]
- juxtaparanode region of axon [IDA, ISO]
- membrane [IDA, ISO]
- neuron projection terminus [IDA]
- neuron spine [IDA]
- neuronal postsynaptic density [IDA, ISO]
- plasma membrane [IDA, ISO]
- postsynaptic density [IDA, ISO]
- postsynaptic membrane [IDA, ISO]
- synapse [IDA, ISO]
- synaptic membrane [IDA]
- synaptic vesicle [IDA]
- voltage-gated potassium channel complex [ISO]
GRIK2
Gene Ontology Biological Process
- behavioral fear response [IMP]
- cellular calcium ion homeostasis [IMP]
- intracellular protein transport [IDA]
- ion transmembrane transport [IGI]
- ionotropic glutamate receptor signaling pathway [IGI, ISO]
- negative regulation of neuron apoptotic process [IGI]
- negative regulation of synaptic transmission, glutamatergic [IGI, ISO]
- neuron apoptotic process [ISO]
- neuronal action potential [IMP]
- positive regulation of neuron apoptotic process [ISO]
- positive regulation of synaptic transmission [ISO]
- receptor clustering [ISO]
- regulation of JNK cascade [ISO]
- regulation of excitatory postsynaptic membrane potential [IGI, IMP]
- regulation of inhibitory postsynaptic membrane potential [IGI, IMP]
- regulation of long-term neuronal synaptic plasticity [IMP]
- regulation of membrane potential [IDA, IGI, IMP]
- regulation of short-term neuronal synaptic plasticity [IMP, ISO]
- regulation of synaptic transmission [ISO]
- signal transduction [ISO]
- synaptic transmission [IGI, IMP, ISO]
- synaptic transmission, glutamatergic [IMP]
Gene Ontology Molecular Function- PDZ domain binding [ISO]
- extracellular-glutamate-gated ion channel activity [IBA]
- glutamate receptor activity [ISO]
- identical protein binding [ISO]
- ionotropic glutamate receptor activity [IMP]
- kainate selective glutamate receptor activity [IDA, IGI, IMP, ISO]
- protein binding [IPI]
- protein homodimerization activity [ISO]
- ubiquitin conjugating enzyme binding [ISO]
- ubiquitin protein ligase binding [ISO]
- PDZ domain binding [ISO]
- extracellular-glutamate-gated ion channel activity [IBA]
- glutamate receptor activity [ISO]
- identical protein binding [ISO]
- ionotropic glutamate receptor activity [IMP]
- kainate selective glutamate receptor activity [IDA, IGI, IMP, ISO]
- protein binding [IPI]
- protein homodimerization activity [ISO]
- ubiquitin conjugating enzyme binding [ISO]
- ubiquitin protein ligase binding [ISO]
Gene Ontology Cellular Component
- axon [ISO]
- cell [IMP]
- dendrite [IBA, ISO]
- dendrite cytoplasm [ISO]
- integral component of plasma membrane [ISO]
- intracellular [IDA]
- ionotropic glutamate receptor complex [IC, ISO]
- kainate selective glutamate receptor complex [IPI]
- membrane [IDA]
- neuronal cell body [IDA]
- perikaryon [ISO]
- postsynaptic density [IDA]
- postsynaptic membrane [IDA]
- presynaptic membrane [IDA]
- synapse [IDA, ISO, TAS]
- terminal bouton [ISO]
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Spatiotemporal profile of postsynaptic interactomes integrates components of complex brain disorders.
The postsynaptic density (PSD) contains a collection of scaffold proteins used for assembling synaptic signaling complexes. However, it is not known how the core-scaffold machinery associates in protein-interaction networks or how proteins encoded by genes involved in complex brain disorders are distributed through spatiotemporal protein complexes. Here using immunopurification, proteomics and bioinformatics, we isolated 2,876 proteins across 41 in vivo ... [more]
Throughput
- High Throughput|Low Throughput
Additional Notes
- Developmental stage: Adult
- Developmental stage: p14
- Postsynaptic density (PSD) scaffold protein Dlg4 was immunoprecipitated and its interactors were identified via mass spec.
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
DLG4 GRIK2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
DLG4 GRIK2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - |
Curated By
- BioGRID