BAIT
DLG4
Dlgh4, PSD-95, PSD95, SAP90, SAP90A, RP23-172M21.9
discs, large homolog 4 (Drosophila)
GO Process (23)
GO Function (18)
GO Component (24)
Gene Ontology Biological Process
- alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor clustering [IBA, ISO]
- dendritic spine morphogenesis [IDA, ISO]
- establishment of protein localization [ISO]
- locomotory behavior [NAS]
- locomotory exploration behavior [IMP]
- negative regulation of receptor internalization [ISO]
- neuromuscular process controlling balance [IMP]
- nucleotide phosphorylation [IBA]
- positive regulation of cytosolic calcium ion concentration [ISO]
- positive regulation of excitatory postsynaptic membrane potential [ISO]
- positive regulation of synaptic transmission [ISO]
- protein complex assembly [ISO]
- protein localization to synapse [IDA, ISO]
- receptor localization to synapse [IBA, ISO]
- regulation of N-methyl-D-aspartate selective glutamate receptor activity [ISO]
- regulation of grooming behavior [IMP]
- regulation of long-term neuronal synaptic plasticity [IGI]
- regulation of neuronal synaptic plasticity [NAS]
- response to cocaine [NAS]
- signal transduction [IBA]
- social behavior [IMP]
- synaptic vesicle maturation [IDA, IGI]
- vocalization behavior [IMP]
Gene Ontology Molecular Function- D1 dopamine receptor binding [ISO]
- P2Y1 nucleotide receptor binding [ISO]
- PDZ domain binding [ISO]
- acetylcholine receptor binding [ISO]
- beta-1 adrenergic receptor binding [ISO]
- glutamate receptor binding [ISO]
- guanylate kinase activity [IBA]
- ionotropic glutamate receptor binding [IPI, ISO]
- kinase binding [ISO]
- neurexin family protein binding [NAS]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein kinase binding [ISO]
- protein phosphatase binding [ISO]
- receptor binding [ISO]
- scaffold protein binding [IPI]
- structural molecule activity [NAS]
- D1 dopamine receptor binding [ISO]
- P2Y1 nucleotide receptor binding [ISO]
- PDZ domain binding [ISO]
- acetylcholine receptor binding [ISO]
- beta-1 adrenergic receptor binding [ISO]
- glutamate receptor binding [ISO]
- guanylate kinase activity [IBA]
- ionotropic glutamate receptor binding [IPI, ISO]
- kinase binding [ISO]
- neurexin family protein binding [NAS]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein kinase binding [ISO]
- protein phosphatase binding [ISO]
- receptor binding [ISO]
- scaffold protein binding [IPI]
- structural molecule activity [NAS]
Gene Ontology Cellular Component
- alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid selective glutamate receptor complex [IDA]
- cell junction [IDA]
- cerebellar mossy fiber [IDA]
- cortical cytoskeleton [ISO]
- cytoplasm [IDA]
- cytosol [ISO]
- dendrite [IDA]
- dendrite cytoplasm [ISO]
- endoplasmic reticulum [IDA]
- excitatory synapse [IDA, ISO]
- extrinsic component of cytoplasmic side of plasma membrane [IDA]
- ionotropic glutamate receptor complex [IDA]
- juxtaparanode region of axon [IDA, ISO]
- membrane [IDA, ISO]
- neuron projection terminus [IDA]
- neuron spine [IDA]
- neuronal postsynaptic density [IDA, ISO]
- plasma membrane [IDA, ISO]
- postsynaptic density [IDA, ISO]
- postsynaptic membrane [IDA, ISO]
- synapse [IDA, ISO]
- synaptic membrane [IDA]
- synaptic vesicle [IDA]
- voltage-gated potassium channel complex [ISO]
Mus musculus
PREY
ANK2
AI835472, AW491075, Ank-2
ankyrin 2, brain
GO Process (33)
GO Function (5)
GO Component (14)
Gene Ontology Biological Process
- SA node cell action potential [IMP]
- SA node cell to atrial cardiac muscle cell communication [IMP]
- T-tubule organization [IMP]
- atrial cardiac muscle cell action potential [IMP]
- atrial cardiac muscle cell to AV node cell communication [IMP]
- cardiac muscle contraction [IMP]
- cellular calcium ion homeostasis [IMP]
- cellular protein localization [IMP]
- positive regulation of calcium ion transmembrane transporter activity [IMP]
- positive regulation of calcium ion transport [IMP]
- positive regulation of cation channel activity [IMP]
- positive regulation of gene expression [IMP]
- positive regulation of potassium ion transmembrane transporter activity [IMP]
- positive regulation of potassium ion transport [IMP]
- protein localization [IMP]
- protein localization to M-band [IMP]
- protein localization to T-tubule [IMP]
- protein localization to cell surface [IMP]
- protein localization to endoplasmic reticulum [IMP]
- protein localization to plasma membrane [IMP]
- protein stabilization [IMP]
- protein targeting to plasma membrane [IGI, IMP]
- regulation of calcium ion transmembrane transporter activity [IMP]
- regulation of calcium ion transport [IMP]
- regulation of cardiac muscle cell contraction [IMP]
- regulation of cardiac muscle cell membrane potential [IGI, IMP]
- regulation of cardiac muscle contraction [IMP]
- regulation of cardiac muscle contraction by calcium ion signaling [IMP]
- regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion [IC, IMP]
- regulation of cation channel activity [IMP]
- regulation of heart rate [IMP]
- regulation of heart rate by cardiac conduction [IMP]
- regulation of protein stability [IC]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- A band [IDA]
- M band [IDA, IMP]
- T-tubule [IDA]
- Z disc [IDA]
- cell [IGI]
- costamere [IDA]
- cytoplasm [IDA]
- integral component of plasma membrane [IDA]
- intercalated disc [IDA]
- intracellular [IGI, IMP]
- neuronal postsynaptic density [IDA]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [IDA, IMP]
- sarcolemma [IDA, IMP]
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Spatiotemporal profile of postsynaptic interactomes integrates components of complex brain disorders.
The postsynaptic density (PSD) contains a collection of scaffold proteins used for assembling synaptic signaling complexes. However, it is not known how the core-scaffold machinery associates in protein-interaction networks or how proteins encoded by genes involved in complex brain disorders are distributed through spatiotemporal protein complexes. Here using immunopurification, proteomics and bioinformatics, we isolated 2,876 proteins across 41 in vivo ... [more]
Nat. Neurosci. Aug. 01, 2017; 20(8);1150-1161 [Pubmed: 28671696]
Throughput
- High Throughput|Low Throughput
Additional Notes
- Developmental stage: p7
- Postsynaptic density (PSD) scaffold protein Dlg4 was immunoprecipitated and its interactors were identified via mass spec.
Curated By
- BioGRID