DLGAP1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SHANK2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- apical plasma membrane [ISO]
- brush border membrane [ISO]
- ciliary membrane [ISO]
- dendritic spine [IDA]
- growth cone [ISO]
- ionotropic glutamate receptor complex [IDA]
- neurofilament [IDA]
- neuron projection [IDA]
- neuronal cell body [ISO]
- photoreceptor inner segment [IDA]
- photoreceptor outer segment [IDA]
- plasma membrane [IDA]
- postsynaptic density [ISO]
- postsynaptic membrane [IDA]
- presynaptic active zone [ISO]
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Spatiotemporal profile of postsynaptic interactomes integrates components of complex brain disorders.
The postsynaptic density (PSD) contains a collection of scaffold proteins used for assembling synaptic signaling complexes. However, it is not known how the core-scaffold machinery associates in protein-interaction networks or how proteins encoded by genes involved in complex brain disorders are distributed through spatiotemporal protein complexes. Here using immunopurification, proteomics and bioinformatics, we isolated 2,876 proteins across 41 in vivo ... [more]
Throughput
- High Throughput
Additional Notes
- Developmental stage: Adult
- Developmental stage: p14
- Developmental stage: p7
- Postsynaptic density (PSD) scaffold protein Dlgap1 was immunoprecipitated and its interactors were identified via mass spec.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| DLGAP1 SHANK2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| SHANK2 DLGAP1 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.723 | BioGRID | 2679723 |
Curated By
- BioGRID