An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Spatiotemporal profile of postsynaptic interactomes integrates components of complex brain disorders.

Li J, Zhang W, Yang H, Howrigan DP, Wilkinson B, Souaiaia T, Evgrafov OV, Genovese G, Clementel VA, Tudor JC, Abel T, Knowles JA, Neale BM, Wang K, Sun F, Coba MP

The postsynaptic density (PSD) contains a collection of scaffold proteins used for assembling synaptic signaling complexes. However, it is not known how the core-scaffold machinery associates in protein-interaction networks or how proteins encoded by genes involved in complex brain disorders are distributed through spatiotemporal protein complexes. Here using immunopurification, proteomics and bioinformatics, we isolated 2,876 proteins across 41 in vivo ... [more]

Nat. Neurosci. Aug. 01, 2017; 20(8);1150-1161 [Pubmed: 28671696]

Throughput

High Throughput

Additional Notes

Developmental stage: Adult
Developmental stage: p14
Developmental stage: p7
Postsynaptic density (PSD) scaffold protein Dlgap1 was immunoprecipitated and its interactors were identified via mass spec.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
DLGAP1 YWHAH	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Li J (2016)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

DLGAP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein complex binding [ISO]protein domain specific binding [ISO]

Gene Ontology Cellular Component

YWHAH

Gene Ontology Biological Process

Gene Ontology Molecular Function

actin binding [IDA]enzyme binding [ISO]glucocorticoid receptor binding [ISO]ion channel binding [IPI, ISO]protein binding [IPI]protein domain specific binding [IDA]protein heterodimerization activity [ISO]sodium channel regulator activity [ISO, ISS]