BAIT
SHANK3
AI841104, SHANK3c-3, SHANK3c-4, Shank3b
SH3/ankyrin domain gene 3
GO Process (33)
GO Function (9)
GO Component (10)
Gene Ontology Biological Process
- MAPK cascade [IGI]
- N-methyl-D-aspartate receptor clustering [IMP]
- adult behavior [ISO]
- alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor clustering [IMP]
- brain morphogenesis [IMP]
- dendritic spine morphogenesis [IMP]
- embryonic epithelial tube formation [IGI]
- guanylate kinase-associated protein clustering [IMP]
- learning [IMP, ISO]
- locomotory exploration behavior [IMP]
- memory [IMP]
- negative regulation of actin filament bundle assembly [IDA]
- negative regulation of cell volume [IMP]
- neuromuscular process controlling balance [IMP]
- positive regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity [IMP]
- positive regulation of dendritic spine development [IMP]
- positive regulation of excitatory postsynaptic membrane potential [IMP]
- positive regulation of glutamate receptor signaling pathway [IMP, ISO]
- positive regulation of long-term neuronal synaptic plasticity [IMP]
- positive regulation of synapse structural plasticity [IMP]
- positive regulation of synaptic transmission, glutamatergic [IMP]
- postsynaptic density assembly [IMP]
- protein oligomerization [ISO]
- regulation of behavioral fear response [IMP]
- regulation of dendritic spine morphogenesis [IMP]
- regulation of grooming behavior [IMP]
- regulation of long term synaptic depression [IMP]
- regulation of long-term synaptic potentiation [IMP]
- social behavior [IMP, ISO]
- striatal medium spiny neuron differentiation [IMP]
- synapse assembly [IMP]
- vocal learning [ISO]
- vocalization behavior [IMP, ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
PLCB1
3110043I21Rik, AI132408, Plcb, mKIAA0581, RP23-418D21.3
phospholipase C, beta 1
GO Process (36)
GO Function (9)
GO Component (8)
Gene Ontology Biological Process
- G-protein coupled acetylcholine receptor signaling pathway [IMP]
- G2/M transition of mitotic cell cycle [IDA, NAS]
- activation of meiosis involved in egg activation [IDA]
- brain development [NAS]
- cell adhesion [IC]
- cerebral cortex development [IMP]
- erythrocyte differentiation [TAS]
- fat cell differentiation [IDA]
- glutamate receptor signaling pathway [IMP]
- insulin receptor signaling pathway [TAS]
- insulin-like growth factor receptor signaling pathway [IDA]
- interleukin-1-mediated signaling pathway [ISO]
- interleukin-12-mediated signaling pathway [ISO]
- interleukin-15-mediated signaling pathway [ISO]
- mRNA processing [TAS]
- macrophage differentiation [NAS]
- memory [IMP]
- negative regulation of monocyte extravasation [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- oocyte maturation [NAS]
- positive regulation of CD24 biosynthetic process [IDA]
- positive regulation of G1/S transition of mitotic cell cycle [IDA]
- positive regulation of GTPase activity [ISO]
- positive regulation of JNK cascade [ISO]
- positive regulation of acrosome reaction [IMP]
- positive regulation of developmental growth [IMP]
- positive regulation of embryonic development [IMP]
- positive regulation of interleukin-12 production [IMP]
- positive regulation of myoblast differentiation [IDA]
- positive regulation of sodium:proton antiporter activity [NAS]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of G-protein coupled receptor protein signaling pathway [IMP]
- regulation of fertilization [IMP]
- response to monosaccharide [ISO]
- response to peptide hormone [ISO]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Spatiotemporal profile of postsynaptic interactomes integrates components of complex brain disorders.
The postsynaptic density (PSD) contains a collection of scaffold proteins used for assembling synaptic signaling complexes. However, it is not known how the core-scaffold machinery associates in protein-interaction networks or how proteins encoded by genes involved in complex brain disorders are distributed through spatiotemporal protein complexes. Here using immunopurification, proteomics and bioinformatics, we isolated 2,876 proteins across 41 in vivo ... [more]
Nat. Neurosci. Aug. 01, 2017; 20(8);1150-1161 [Pubmed: 28671696]
Throughput
- High Throughput
Additional Notes
- Developmental stage: Adult
- Developmental stage: p14
- Developmental stage: p7
- Postsynaptic density (PSD) scaffold protein Shank3 was immunoprecipitated and its interactors were identified via mass spec.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SHANK3 PLCB1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - |
Curated By
- BioGRID