BAIT
SHANK3
AI841104, SHANK3c-3, SHANK3c-4, Shank3b
SH3/ankyrin domain gene 3
GO Process (33)
GO Function (9)
GO Component (10)
Gene Ontology Biological Process
- MAPK cascade [IGI]
- N-methyl-D-aspartate receptor clustering [IMP]
- adult behavior [ISO]
- alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor clustering [IMP]
- brain morphogenesis [IMP]
- dendritic spine morphogenesis [IMP]
- embryonic epithelial tube formation [IGI]
- guanylate kinase-associated protein clustering [IMP]
- learning [IMP, ISO]
- locomotory exploration behavior [IMP]
- memory [IMP]
- negative regulation of actin filament bundle assembly [IDA]
- negative regulation of cell volume [IMP]
- neuromuscular process controlling balance [IMP]
- positive regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity [IMP]
- positive regulation of dendritic spine development [IMP]
- positive regulation of excitatory postsynaptic membrane potential [IMP]
- positive regulation of glutamate receptor signaling pathway [IMP, ISO]
- positive regulation of long-term neuronal synaptic plasticity [IMP]
- positive regulation of synapse structural plasticity [IMP]
- positive regulation of synaptic transmission, glutamatergic [IMP]
- postsynaptic density assembly [IMP]
- protein oligomerization [ISO]
- regulation of behavioral fear response [IMP]
- regulation of dendritic spine morphogenesis [IMP]
- regulation of grooming behavior [IMP]
- regulation of long term synaptic depression [IMP]
- regulation of long-term synaptic potentiation [IMP]
- social behavior [IMP, ISO]
- striatal medium spiny neuron differentiation [IMP]
- synapse assembly [IMP]
- vocal learning [ISO]
- vocalization behavior [IMP, ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
MAPK3
Erk-1, Erk1, Ert2, Esrk1, Mnk1, Mtap2k, Prkm3, p44, p44erk1, p44mapk
mitogen-activated protein kinase 3
GO Process (35)
GO Function (6)
GO Component (15)
Gene Ontology Biological Process
- BMP signaling pathway [ISO]
- DNA damage induced protein phosphorylation [ISO]
- MAPK cascade [IMP, ISO]
- MAPK import into nucleus [ISO]
- cartilage development [IDA]
- caveolin-mediated endocytosis [TAS]
- cellular response to DNA damage stimulus [IDA]
- interleukin-1-mediated signaling pathway [ISO]
- intracellular signal transduction [ISO]
- lipopolysaccharide-mediated signaling pathway [IDA]
- negative regulation of apolipoprotein binding [IMP]
- organ morphogenesis [IDA]
- peptidyl-serine phosphorylation [ISO]
- peptidyl-tyrosine autophosphorylation [ISO]
- phosphorylation [ISO, TAS]
- positive regulation of ERK1 and ERK2 cascade [ISO]
- positive regulation of histone acetylation [ISO]
- positive regulation of histone phosphorylation [ISO]
- positive regulation of protein phosphorylation [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of translation [ISO]
- protein complex assembly [ISO]
- protein phosphorylation [IDA, IMP, ISO]
- regulation of Golgi inheritance [TAS]
- regulation of cytoskeleton organization [TAS]
- regulation of early endosome to late endosome transport [TAS]
- regulation of sequence-specific DNA binding transcription factor activity [IMP]
- regulation of stress-activated MAPK cascade [TAS]
- response to epidermal growth factor [ISO]
- response to exogenous dsRNA [IDA]
- response to lipopolysaccharide [IDA]
- response to toxic substance [ISO]
- sensory perception of pain [IMP]
- signal transduction [TAS]
- transcription, DNA-templated [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [TAS]
- caveola [TAS]
- cytoplasm [IDA]
- cytoskeleton [TAS]
- cytosol [IDA, ISO, TAS]
- early endosome [TAS]
- extracellular vesicular exosome [ISO]
- focal adhesion [TAS]
- late endosome [TAS]
- microtubule cytoskeleton [ISO]
- mitochondrion [TAS]
- nucleoplasm [ISO]
- nucleus [IDA, ISO, TAS]
- protein complex [ISO]
- pseudopodium [IDA]
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Spatiotemporal profile of postsynaptic interactomes integrates components of complex brain disorders.
The postsynaptic density (PSD) contains a collection of scaffold proteins used for assembling synaptic signaling complexes. However, it is not known how the core-scaffold machinery associates in protein-interaction networks or how proteins encoded by genes involved in complex brain disorders are distributed through spatiotemporal protein complexes. Here using immunopurification, proteomics and bioinformatics, we isolated 2,876 proteins across 41 in vivo ... [more]
Nat. Neurosci. Aug. 01, 2017; 20(8);1150-1161 [Pubmed: 28671696]
Throughput
- High Throughput
Additional Notes
- Developmental stage: p14
- Postsynaptic density (PSD) scaffold protein Shank3 was immunoprecipitated and its interactors were identified via mass spec.
Curated By
- BioGRID