HMG2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CDC48
Gene Ontology Biological Process
- ER-associated misfolded protein catabolic process [IMP]
- ER-associated ubiquitin-dependent protein catabolic process [IMP]
- SCF complex disassembly in response to cadmium stress [IMP]
- cytoplasm-associated proteasomal ubiquitin-dependent protein catabolic process [IMP]
- endoplasmic reticulum membrane fusion [IMP]
- macroautophagy [IMP]
- mitochondria-associated ubiquitin-dependent protein catabolic process [IMP]
- mitotic spindle disassembly [IMP]
- nonfunctional rRNA decay [IMP]
- nucleus-associated proteasomal ubiquitin-dependent protein catabolic process [IMP]
- piecemeal microautophagy of nucleus [IMP]
- positive regulation of histone H2B ubiquitination [IMP]
- positive regulation of protein localization to nucleus [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IMP]
- retrograde protein transport, ER to cytosol [IMP]
- ribophagy [IMP]
- ribosome-associated ubiquitin-dependent protein catabolic process [IMP]
- sister chromatid biorientation [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Cdc48p-Npl4p-Ufd1p AAA ATPase complex [IDA]
- Cdc48p-Npl4p-Vms1p AAA ATPase complex [IDA]
- Doa10p ubiquitin ligase complex [IDA]
- Hrd1p ubiquitin ligase ERAD-L complex [IDA]
- RQC complex [IDA]
- cytosol [IDA]
- cytosolic large ribosomal subunit [IDA]
- endoplasmic reticulum membrane [IDA]
- mating projection tip [IDA]
- mitochondrion [IDA]
- nucleus [IDA]
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A Cdc48 "Retrochaperone" Function Is Required for the Solubility of Retrotranslocated, Integral Membrane Endoplasmic Reticulum-associated Degradation (ERAD-M) Substrates.
A surprising feature of endoplasmic reticulum (ER)-associated degradation (ERAD) is the movement, or retrotranslocation, of ubiquitinated substrates from the ER lumen or membrane to the cytosol where they are degraded by the 26S proteasome. Multispanning ER membrane proteins, called ERAD-M substrates, are retrotranslocated to the cytosol as full-length intermediates during ERAD, and we have investigated how they maintain substrate solubility. ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| HMG2 CDC48 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CDC48 HMG2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| HMG2 CDC48 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| HMG2 CDC48 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| HMG2 CDC48 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID