MPHOSPH6
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
EXOSC9
Gene Ontology Biological Process
- RNA metabolic process [TAS]
- RNA phosphodiester bond hydrolysis, exonucleolytic [NAS]
- exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay [IMP, TAS]
- gene expression [TAS]
- immune response [NAS]
- mRNA metabolic process [TAS]
- nuclear mRNA surveillance [IMP]
- nuclear polyadenylation-dependent rRNA catabolic process [IMP]
- nuclear-transcribed mRNA catabolic process [IMP]
- nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [TAS]
- positive regulation of cell growth [IMP]
- rRNA processing [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Mpp6 Incorporation in the Nuclear Exosome Contributes to RNA Channeling through the Mtr4 Helicase.
The RNA-degrading exosome mediates the processing and decay of many cellular transcripts. In the yeast nucleus, the ubiquitous 10-subunit exosome core complex (Exo-9-Rrp44) functions with four conserved cofactors (Rrp6, Rrp47, Mtr4, and Mpp6). Biochemical and structural studies to date have shed insights into the mechanisms of the exosome core and its nuclear cofactors, with the exception of Mpp6. We report ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| EXOSC9 MPHOSPH6 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 1450327 | |
| MPHOSPH6 EXOSC9 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9434 | BioGRID | 3069253 | |
| MPHOSPH6 EXOSC9 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 102.9117 | BioGRID | 2941752 | |
| EXOSC9 MPHOSPH6 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | - | BioGRID | 3448797 | |
| EXOSC9 MPHOSPH6 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 1 | BioGRID | 1264729 | |
| EXOSC9 MPHOSPH6 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -4.1091 | BioGRID | 2457209 |
Curated By
- BioGRID