BAIT
ISW1
SGN2, chromatin-remodeling ATPase ISW1, L000004447, YBR245C
ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; with Ioc3p forms Isw1a complex involved in repression of transcription initiation; with Ioc2p and Ioc4p forms Isw1b complex involved in regulation of transcription elongation; Isw1b recruited to ORFs by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions; Isw1p import into nucleus depends on C-terminal bipartite nuclear targeting signal KRIR X19 KKAK
GO Process (11)
GO Function (4)
GO Component (4)
Gene Ontology Biological Process
- DNA-templated transcription, elongation [IDA, IMP]
- chromatin organization involved in regulation of transcription [IMP]
- chromatin remodeling [IGI, IMP, IPI]
- heterochromatin maintenance involved in chromatin silencing [IGI, IMP]
- negative regulation of histone exchange [IMP]
- nucleosome positioning [IGI, IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of chromatin organization [IMP]
- regulation of transcriptional start site selection at RNA polymerase II promoter [IGI]
- termination of RNA polymerase I transcription [IGI]
- termination of RNA polymerase II transcription [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
NGL3
YML118W
3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
GO Process (1)
GO Function (2)
GO Component (0)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Affinity Capture-RNA
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.
Publication
The Chromatin Remodeler ISW1 Is a Quality Control Factor that Surveys Nuclear mRNP Biogenesis.
Chromatin dynamics play an essential role in regulating DNA transaction processes, but it is unclear whether transcription-associated chromatin modifications control the mRNA ribonucleoparticles (mRNPs) pipeline from synthesis to nuclear exit. Here, we identify the yeast ISW1 chromatin remodeling complex as an unanticipated mRNP nuclear export surveillance factor that retains export-incompetent transcripts near their transcription site. This tethering activity of ISW1 ... [more]
Cell Dec. 17, 2015; 167(5);1201-1214.e15 [Pubmed: 27863241]
Throughput
- High Throughput
Additional Notes
- in npl3 mutant background
Curated By
- BioGRID