BAIT

RRN3

L000003362, YKL125W
Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo
Saccharomyces cerevisiae (S288c)
PREY

RPC19

DNA-directed RNA polymerase core subunit RPC19, AC19, L000001687, YNL113W
RNA polymerase subunit AC19; common to RNA polymerases I and III
Saccharomyces cerevisiae (S288c)

Co-crystal Structure

Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.

Publication

The dynamic assembly of distinct RNA polymerase I complexes modulates rDNA transcription.

Torreira E, Louro JA, Pazos I, Gonzalez-Polo N, Gil-Carton D, Duran AG, Tosi S, Gallego O, Calvo O, Fernandez-Tornero C

Cell growth requires synthesis of ribosomal RNA by RNA polymerase I (Pol I). Binding of initiation factor Rrn3 activates Pol I, fostering recruitment to ribosomal DNA promoters. This fundamental process must be precisely regulated to satisfy cell needs at any time. We present in vivo evidence that, when growth is arrested by nutrient deprivation, cells induce rapid clearance of Pol ... [more]

Elife Mar. 06, 2017; 6(); [Pubmed: 28262097]

Throughput

  • Low Throughput

Additional Notes

  • cryo-EM

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RPC19 RRN3
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.343BioGRID
1948741

Curated By

  • BioGRID