BAIT
CNKSR2
C030004A01, Cnk2, Ksr2, mKIAA0902, RP23-93M14.3
connector enhancer of kinase suppressor of Ras 2
GO Process (1)
GO Function (2)
GO Component (7)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
SYT1
AW124717, G630098F17Rik, SytI
synaptotagmin I
GO Process (9)
GO Function (15)
GO Component (13)
Gene Ontology Biological Process
- detection of calcium ion [ISO]
- neurotransmitter secretion [IDA]
- positive regulation of calcium ion-dependent exocytosis [ISO]
- positive regulation of vesicle fusion [ISO]
- regulation of calcium ion-dependent exocytosis [ISO]
- regulation of regulated secretory pathway [ISO]
- response to calcium ion [ISO]
- synaptic vesicle exocytosis [IMP]
- vesicle docking [ISO]
Gene Ontology Molecular Function- SNARE binding [ISO]
- calcium ion binding [IDA, ISO]
- calcium-dependent phospholipid binding [ISO, TAS]
- calcium-dependent protein binding [IPI, ISO]
- calmodulin binding [ISO]
- clathrin binding [ISO]
- identical protein binding [ISO]
- low-density lipoprotein particle receptor binding [ISO]
- phosphatidylinositol-4,5-bisphosphate binding [ISO]
- phosphatidylserine binding [ISO]
- phospholipid binding [ISO]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- syntaxin binding [ISO]
- syntaxin-1 binding [ISO]
- SNARE binding [ISO]
- calcium ion binding [IDA, ISO]
- calcium-dependent phospholipid binding [ISO, TAS]
- calcium-dependent protein binding [IPI, ISO]
- calmodulin binding [ISO]
- clathrin binding [ISO]
- identical protein binding [ISO]
- low-density lipoprotein particle receptor binding [ISO]
- phosphatidylinositol-4,5-bisphosphate binding [ISO]
- phosphatidylserine binding [ISO]
- phospholipid binding [ISO]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- syntaxin binding [ISO]
- syntaxin-1 binding [ISO]
Gene Ontology Cellular Component
- SNARE complex [ISO]
- cell [IMP]
- cytoplasm [ISO]
- dense core granule [ISO]
- excitatory synapse [ISO]
- intracellular organelle [ISO]
- neuron projection [IDA]
- plasma membrane [ISO, TAS]
- presynaptic membrane [IDA]
- secretory granule [IDA, ISO]
- synaptic vesicle [IDA, ISO, TAS]
- synaptic vesicle membrane [IDA]
- terminal bouton [ISO]
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Spatiotemporal profile of postsynaptic interactomes integrates components of complex brain disorders.
The postsynaptic density (PSD) contains a collection of scaffold proteins used for assembling synaptic signaling complexes. However, it is not known how the core-scaffold machinery associates in protein-interaction networks or how proteins encoded by genes involved in complex brain disorders are distributed through spatiotemporal protein complexes. Here using immunopurification, proteomics and bioinformatics, we isolated 2,876 proteins across 41 in vivo ... [more]
Nat. Neurosci. Aug. 01, 2017; 20(8);1150-1161 [Pubmed: 28671696]
Throughput
- High Throughput
Additional Notes
- Adult-non postsynaptic density (PSD)
Curated By
- BioGRID