An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Spatiotemporal profile of postsynaptic interactomes integrates components of complex brain disorders.

Li J, Zhang W, Yang H, Howrigan DP, Wilkinson B, Souaiaia T, Evgrafov OV, Genovese G, Clementel VA, Tudor JC, Abel T, Knowles JA, Neale BM, Wang K, Sun F, Coba MP

The postsynaptic density (PSD) contains a collection of scaffold proteins used for assembling synaptic signaling complexes. However, it is not known how the core-scaffold machinery associates in protein-interaction networks or how proteins encoded by genes involved in complex brain disorders are distributed through spatiotemporal protein complexes. Here using immunopurification, proteomics and bioinformatics, we isolated 2,876 proteins across 41 in vivo ... [more]

Nat. Neurosci. Aug. 01, 2017; 20(8);1150-1161 [Pubmed: 28671696]

Throughput

High Throughput

Additional Notes

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TNIK HERC2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Li J (2017)	High	-	BioGRID	2343877

Curated By

BioGRID

Interaction Summary

HERC2

Gene Ontology Biological Process

Gene Ontology Molecular Function

SUMO binding [ISO]ubiquitin protein ligase binding [ISO]ubiquitin-protein transferase activity [IBA, ISO]

Gene Ontology Cellular Component

TNIK

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein serine/threonine kinase activity [ISO]receptor signaling protein serine/threonine kinase activity [IBA]