BAIT
DHFR
DHFRP1, DYR
dihydrofolate reductase
GO Process (10)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [TAS]
- folic acid metabolic process [TAS]
- mitotic cell cycle [TAS]
- nitric oxide metabolic process [TAS]
- regulation of nitric-oxide synthase activity [TAS]
- regulation of transcription involved in G1/S transition of mitotic cell cycle [TAS]
- small molecule metabolic process [TAS]
- tetrahydrofolate metabolic process [IDA]
- vitamin metabolic process [TAS]
- water-soluble vitamin metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cytosol [TAS]
- nucleoplasm [TAS]
Homo sapiens
PREY
CHEK1
CHK1
checkpoint kinase 1
GO Process (15)
GO Function (3)
GO Component (9)
Gene Ontology Biological Process
- DNA damage checkpoint [IDA, IMP]
- DNA damage induced protein phosphorylation [IDA]
- DNA repair [IMP]
- DNA replication [TAS]
- G2 DNA damage checkpoint [IMP]
- cellular response to DNA damage stimulus [IMP]
- cellular response to mechanical stimulus [IEP]
- chromatin-mediated maintenance of transcription [ISS]
- negative regulation of mitosis [IDA]
- peptidyl-threonine phosphorylation [IDA]
- regulation of double-strand break repair via homologous recombination [IDA]
- regulation of histone H3-K9 acetylation [ISS]
- regulation of mitotic centrosome separation [IDA]
- regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage [ISS]
- replicative senescence [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
A Network of Conserved Synthetic Lethal Interactions for Exploration of Precision Cancer Therapy.
An emerging therapeutic strategy for cancer is to induce selective lethality in a tumor by exploiting interactions between its driving mutations and specific drug targets. Here we use a multi-species approach to develop a resource of synthetic lethal interactions relevant to cancer therapy. First, we screen in yeast ∼169,000 potential interactions among orthologs of human tumor suppressor genes (TSG) and ... [more]
Mol. Cell Aug. 04, 2016; 63(3);514-25 [Pubmed: 27453043]
Throughput
- High Throughput
Ontology Terms
- growth abnormality (HP:0001507) [hela cell (BTO:0000567)]
Additional Notes
- Chemo-genetic screen with siRNAs
- Drug: methotrexate
- HeLa cervical cancer cell line
Curated By
- BioGRID