An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Nuclear Functions of Nucleolin through Global Proteomics and Interactomic Approaches.

Salvetti A, Coute Y, Epstein A, Arata L, Kraut A, Navratil V, Bouvet P, Greco A

Nucleolin (NCL) is a major component of the cell nucleolus, which has the ability to rapidly shuttle to several other cells' compartments. NCL plays important roles in a variety of essential functions, among which are ribosome biogenesis, gene expression, and cell growth. However, the precise mechanisms underlying NCL functions are still unclear. Our study aimed to provide new information on ... [more]

J. Proteome Res. May. 06, 2016; 15(5);1659-69 [Pubmed: 27049334]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

NCL

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA binding [IDA]identical protein binding [IPI]poly(A) RNA binding [IDA]protein C-terminus binding [IPI]protein binding [IPI]telomeric DNA binding [IDA]

Gene Ontology Cellular Component

SLC25A1