BAIT
TNIK
1500031A17Rik, 4831440I19Rik, AI451411, C530008O15Rik, C630040K21Rik
TRAF2 and NCK interacting kinase
GO Process (9)
GO Function (3)
GO Component (7)
Gene Ontology Biological Process
- actin cytoskeleton reorganization [ISO]
- activation of JNKK activity [ISO]
- cytoskeleton organization [ISO]
- intracellular signal transduction [IBA, ISO]
- protein autophosphorylation [ISO]
- protein phosphorylation [IBA, ISO]
- regulation of dendrite morphogenesis [ISO]
- response to stress [IBA]
- signal transduction by phosphorylation [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
CASK
DXPri1, DXRib1, LIN-2, Pals3, mLin-2, RP23-278L4.1
calcium/calmodulin-dependent serine protein kinase (MAGUK family)
GO Process (8)
GO Function (5)
GO Component (18)
Gene Ontology Biological Process
- calcium ion import [IMP]
- intracellular protein transport [ISO]
- negative regulation of cell-matrix adhesion [ISO]
- negative regulation of cellular response to growth factor stimulus [ISO]
- negative regulation of keratinocyte proliferation [ISO]
- negative regulation of wound healing [ISO]
- positive regulation of calcium ion import [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- basement membrane [IDA, ISO]
- basolateral plasma membrane [IDA]
- cell-cell junction [IDA, ISO]
- ciliary membrane [ISO]
- cytoplasm [IDA, ISO]
- cytosol [IDA]
- dendrite [ISO]
- focal adhesion [ISO]
- membrane [IDA, ISO]
- nuclear lamina [ISO]
- nuclear matrix [ISO]
- nucleolus [ISO]
- nucleus [IDA, ISO]
- plasma membrane [ISO]
- presynaptic membrane [ISO]
- protein complex [ISO]
- synapse [IDA]
- synaptic membrane [ISO]
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Spatiotemporal profile of postsynaptic interactomes integrates components of complex brain disorders.
The postsynaptic density (PSD) contains a collection of scaffold proteins used for assembling synaptic signaling complexes. However, it is not known how the core-scaffold machinery associates in protein-interaction networks or how proteins encoded by genes involved in complex brain disorders are distributed through spatiotemporal protein complexes. Here using immunopurification, proteomics and bioinformatics, we isolated 2,876 proteins across 41 in vivo ... [more]
Nat. Neurosci. Aug. 01, 2017; 20(8);1150-1161 [Pubmed: 28671696]
Throughput
- High Throughput
Additional Notes
- Adult non-postsynaptic density (PSD)
- Adult postsynaptic density (PSD)
- e14
Curated By
- BioGRID