An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Evolution of an intricate J-protein network driving protein disaggregation in eukaryotes.

Nillegoda NB, Stank A, Malinverni D, Alberts N, Szlachcic A, Barducci A, De Los Rios P, Wade RC, Bukau B

Hsp70 participates in a broad spectrum of protein folding processes extending from nascent chain folding to protein disaggregation. This versatility in function is achieved through a diverse family of J-protein cochaperones that select substrates for Hsp70. Substrate selection is further tuned by transient complexation between different classes of J-proteins, which expands the range of protein aggregates targeted by metazoan Hsp70 ... [more]

Elife May. 15, 2017; 6(); [Pubmed: 28504929]

Throughput

Low Throughput

Additional Notes

in situ PLA

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
DNAJA2 DNAJB1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Piette BL (2021)	High	150	BioGRID	2937014
DNAJA2 DNAJB1	Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.	Nillegoda NB (2017)	Low	-	BioGRID	2344299

Curated By

BioGRID

Interaction Summary

DNAJA2

Gene Ontology Biological Process

Gene Ontology Molecular Function

chaperone binding [IPI]protein binding [IPI]unfolded protein binding [IDA]

Gene Ontology Cellular Component

DNAJB1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATPase activator activity [IDA]ATPase binding [IPI]Hsp70 protein binding [IPI]chaperone binding [IPI]protein binding [IPI]unfolded protein binding [IDA]