HSPA4
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
DNAJB2
Gene Ontology Biological Process
- ER-associated ubiquitin-dependent protein catabolic process [IDA]
- negative regulation of cell growth [IGI]
- negative regulation of cell proliferation [IGI]
- negative regulation of inclusion body assembly [IDA]
- negative regulation of protein deubiquitination [IDA]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IDA]
- positive regulation of protein ubiquitination [IDA]
- protein folding [TAS]
- protein refolding [IDA]
- response to unfolded protein [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Co-localization
Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.
Publication
Evolution of an intricate J-protein network driving protein disaggregation in eukaryotes.
Hsp70 participates in a broad spectrum of protein folding processes extending from nascent chain folding to protein disaggregation. This versatility in function is achieved through a diverse family of J-protein cochaperones that select substrates for Hsp70. Substrate selection is further tuned by transient complexation between different classes of J-proteins, which expands the range of protein aggregates targeted by metazoan Hsp70 ... [more]
Throughput
- Low Throughput
Additional Notes
- in situ PLA
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| DNAJB2 HSPA4 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 4.36 | BioGRID | 2937134 | |
| DNAJB2 HSPA4 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| DNAJB2 HSPA4 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 2394191 |
Curated By
- BioGRID