Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

Evolution of an intricate J-protein network driving protein disaggregation in eukaryotes.

Nillegoda NB, Stank A, Malinverni D, Alberts N, Szlachcic A, Barducci A, De Los Rios P, Wade RC, Bukau B

Hsp70 participates in a broad spectrum of protein folding processes extending from nascent chain folding to protein disaggregation. This versatility in function is achieved through a diverse family of J-protein cochaperones that select substrates for Hsp70. Substrate selection is further tuned by transient complexation between different classes of J-proteins, which expands the range of protein aggregates targeted by metazoan Hsp70 ... [more]

Elife May. 15, 2017; 6(); [Pubmed: 28504929]

Throughput

Low Throughput

Additional Notes

in situ PLA

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
DNAJB8 HSPA4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Piette BL (2021)	High	4.47	BioGRID	2937236

Curated By

BioGRID

Interaction Summary

HSPA4

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [NAS]

Gene Ontology Cellular Component

DNAJB8