CYLD
Gene Ontology Biological Process
- cytoplasmic translation [IBA]
- innate immune response [TAS]
- necroptotic process [IBA]
- negative regulation of NF-kappaB import into nucleus [IDA]
- negative regulation of NF-kappaB transcription factor activity [IDA]
- negative regulation of canonical Wnt signaling pathway [IMP]
- negative regulation of type I interferon production [TAS]
- nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway [TAS]
- nucleotide-binding oligomerization domain containing signaling pathway [TAS]
- positive regulation of extrinsic apoptotic signaling pathway [IMP]
- protein K63-linked deubiquitination [IDA]
- regulation of cilium assembly [ISS]
- regulation of intrinsic apoptotic signaling pathway [IMP]
- regulation of microtubule cytoskeleton organization [IMP]
- regulation of mitotic cell cycle [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
TRAF2
Gene Ontology Biological Process
- activation of NF-kappaB-inducing kinase activity [IMP]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [TAS]
- apoptotic process [TAS]
- apoptotic signaling pathway [TAS]
- cellular protein complex assembly [ISS]
- innate immune response [TAS]
- negative regulation of neuron death [TAS]
- positive regulation of JUN kinase activity [IDA]
- positive regulation of NF-kappaB transcription factor activity [IDA, IMP]
- positive regulation of T cell activation [IC]
- positive regulation of T cell cytokine production [IMP]
- positive regulation of extrinsic apoptotic signaling pathway [IMP]
- positive regulation of interleukin-2 production [IMP]
- positive regulation of protein homodimerization activity [IMP]
- positive regulation of sequence-specific DNA binding transcription factor activity [IMP]
- protein K63-linked ubiquitination [IDA]
- protein autoubiquitination [IDA, TAS]
- protein complex assembly [TAS]
- protein homotrimerization [IPI]
- regulation of apoptotic process [IDA]
- regulation of extrinsic apoptotic signaling pathway in absence of ligand [TAS]
- signal transduction [TAS]
- tumor necrosis factor-mediated signaling pathway [IDA]
Gene Ontology Molecular Function- CD40 receptor binding [ISS]
- enzyme binding [IPI]
- identical protein binding [IPI]
- protein binding [IPI]
- protein phosphatase binding [IPI]
- signal transducer activity [NAS]
- sphingolipid binding [IDA]
- thioesterase binding [IPI]
- tumor necrosis factor receptor binding [IPI]
- ubiquitin protein ligase binding [IPI]
- ubiquitin-protein transferase activity [IDA]
- CD40 receptor binding [ISS]
- enzyme binding [IPI]
- identical protein binding [IPI]
- protein binding [IPI]
- protein phosphatase binding [IPI]
- signal transducer activity [NAS]
- sphingolipid binding [IDA]
- thioesterase binding [IPI]
- tumor necrosis factor receptor binding [IPI]
- ubiquitin protein ligase binding [IPI]
- ubiquitin-protein transferase activity [IDA]
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
SPATA2 Links CYLD to LUBAC, Activates CYLD, and Controls LUBAC Signaling.
The linear ubiquitin chain assembly complex (LUBAC) regulates immune signaling, and its function is regulated by the deubiquitinases OTULIN and CYLD, which associate with the catalytic subunit HOIP. However, the mechanism through which CYLD interacts with HOIP is unclear. We here show that CYLD interacts with HOIP via spermatogenesis-associated protein 2 (SPATA2). SPATA2 interacts with CYLD through its non-canonical PUB ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CYLD TRAF2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| TRAF2 CYLD | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| TRAF2 CYLD | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CYLD TRAF2 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 503872 | |
| TRAF2 CYLD | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| CYLD TRAF2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID