Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The histone-fold complex MHF is remodeled by FANCM to recognize branched DNA and protect genome stability.

Fox D, Yan Z, Ling C, Zhao Y, Lee DY, Fukagawa T, Yang W, Wang W

Histone-fold proteins typically assemble in multiprotein complexes to bind duplex DNA. However, one histone-fold complex, MHF, associates with Fanconi anemia (FA) protein FANCM to form a branched DNA remodeling complex that senses and repairs stalled replication forks and activates FA DNA damage response network. How the FANCM-MHF complex recognizes branched DNA is unclear. Here, we solved the crystal structure of ... [more]

Cell Res. May. 01, 2014; 24(5);560-75 [Pubmed: 24699063]

Throughput

  • Low Throughput

Curated By

  • BioGRID