MCM5
Gene Ontology Biological Process
- DNA replication initiation [IMP]
- chromatin silencing at telomere [IMP]
- double-strand break repair via break-induced replication [IMP]
- establishment of chromatin silencing [IMP]
- negative regulation of chromatin silencing at telomere [IMP]
- nuclear DNA replication [IMP]
- pre-replicative complex assembly involved in nuclear cell cycle DNA replication [IDA, IPI]
- regulation of DNA-dependent DNA replication initiation [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
DPB11
Gene Ontology Biological Process
- DNA replication checkpoint [IMP]
- DNA replication initiation [IMP]
- double-strand break repair via break-induced replication [IMP]
- lagging strand elongation [TAS]
- leading strand elongation [TAS]
- mating type switching [IMP]
- mismatch repair [NAS]
- mitotic G2 DNA damage checkpoint [IGI]
- nucleotide-excision repair [TAS]
- positive regulation of protein phosphorylation [IDA]
- recombinational repair [IDA, IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Dpb11 protein helps control assembly of the Cdc45·Mcm2-7·GINS replication fork helicase.
Dpb11 is required for the initiation of DNA replication in budding yeast. Dpb11 binds to S-phase cyclin-dependent kinase-phosphorylated Sld2 and Sld3 to form a ternary complex during S phase. The replication fork helicase in eukaryotes is composed of Cdc45, Mcm2-7, and GINS. We show here, using purified proteins from budding yeast, that Dpb11 alone binds to Mcm2-7 and that Dpb11 ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| DPB11 MCM5 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.3114 | BioGRID | 1939220 | |
| MCM5 DPB11 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.1737 | BioGRID | 1944657 |
Curated By
- BioGRID