BAIT

ARL3

ARFL3, RP11-47A8.3
ADP-ribosylation factor-like 3
Homo sapiens
PREY

RP2

DELXp11.3, NM23-H10, NME10, TBCCD2, XRP2
retinitis pigmentosa 2 (X-linked recessive)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Crystal structure of the human retinitis pigmentosa 2 protein and its interaction with Arl3.

Kuehnel K, Veltel S, Schlichting I, Wittinghofer A

The crystal structure of human retinitis pigmentosa 2 protein (RP2) was solved to 2.1 angstroms resolution. It consists of an N-terminal beta helix and a C-terminal ferredoxin-like alpha/beta domain. RP2 is functionally and structurally related to the tubulin-specific chaperone cofactor C. Seven of nine known RP2 missense mutations identified in patients are located in the beta helix domain, and most ... [more]

Structure Feb. 01, 2006; 14(2);367-78 [Pubmed: 16472755]

Throughput

  • Low Throughput

Additional Notes

  • Actual experiment was a fluorescence polarization assay. This involved purified proteins tagged with a fluorescence molecule being added together in vitro, the association of the two proteins changed the polarization of the fluorescence which could be detected.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RP2 ARL3
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
-

Curated By

  • BioGRID