MCM6
Gene Ontology Biological Process
- DNA replication initiation [IPI]
- DNA strand elongation involved in DNA replication [IMP]
- DNA unwinding involved in DNA replication [IDA]
- double-strand break repair via break-induced replication [IMP]
- nuclear DNA replication [IMP]
- pre-replicative complex assembly involved in nuclear cell cycle DNA replication [IDA]
Gene Ontology Molecular Function- ATP-dependent 3'-5' DNA helicase activity [IDA]
- ATP-dependent DNA helicase activity [IDA]
- ATP-dependent four-way junction helicase activity [IDA]
- DNA helicase activity [IDA]
- DNA replication origin binding [IDA]
- single-stranded DNA-dependent ATP-dependent DNA helicase activity [IDA]
- single-stranded DNA-dependent ATPase activity [IDA]
- ATP-dependent 3'-5' DNA helicase activity [IDA]
- ATP-dependent DNA helicase activity [IDA]
- ATP-dependent four-way junction helicase activity [IDA]
- DNA helicase activity [IDA]
- DNA replication origin binding [IDA]
- single-stranded DNA-dependent ATP-dependent DNA helicase activity [IDA]
- single-stranded DNA-dependent ATPase activity [IDA]
Gene Ontology Cellular Component
CSM3
Gene Ontology Biological Process
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Mcm2-7 Is an Active Player in the DNA Replication Checkpoint Signaling Cascade via Proposed Modulation of Its DNA Gate.
The DNA replication checkpoint (DRC) monitors and responds to stalled replication forks to prevent genomic instability. How core replication factors integrate into this phosphorylation cascade is incompletely understood. Here, through analysis of a unique mcm allele targeting a specific ATPase active site (mcm2DENQ), we show that the Mcm2-7 replicative helicase has a novel DRC function as part of the signal ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CSM3 MCM6 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| MCM6 CSM3 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.2239 | BioGRID | 1983419 |
Curated By
- BioGRID