BAIT
DHH1
DExD/H-box ATP-dependent RNA helicase DHH1, L000000504, YDL160C
Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, role in translational repression, mRNA decay, and processing body dynamics; may have a role in mRNA export; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress
GO Process (5)
GO Function (5)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
MSN1
FUP1, HRB382, MSS10, PHD2, L000001197, YOL116W
Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress
GO Process (6)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
- positive regulation of invasive growth in response to glucose limitation by positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of pseudohyphal growth by positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of starch catabolic process by positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription from RNA polymerase II promoter in response to heat stress [IMP]
- positive regulation of transcription from RNA polymerase II promoter in response to increased salt [IGI, IMP]
- positive regulation of transcription from RNA polymerase II promoter in response to methylglyoxal [IMP]
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Affinity Capture-RNA
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.
Publication
Genome-Wide Mapping of Decay Factor-mRNA Interactions in Yeast Identifies Nutrient Responsive Transcripts as Targets of the Deadenylase Ccr4.
The Ccr4-Not complex is a major regulator of stress responses that controls gene expression at multiple levels, from transcription to mRNA decay. Ccr4, a core subunit of the complex, is the main cytoplasmic deadenylase in Saccharomyces cerevisiae, however its mRNA targets have not been mapped on a genome-wide scale. Here we describe a genome-wide approach, RNA immunoprecipitation-high throughput sequencing (RIP-seq), ... [more]
G3 (Bethesda) Nov. 20, 2017; (); [Pubmed: 29158339]
Throughput
- High Throughput
Curated By
- BioGRID