BAIT
MSH1
mismatch repair ATPase MSH1, L000001189, YHR120W
DNA-binding protein of the mitochondria; involved in repair of mitochondrial DNA; has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis
GO Process (8)
GO Function (5)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
POL2
DUN2, DNA polymerase epsilon catalytic subunit, L000001461, YNL262W
Catalytic subunit of DNA polymerase (II) epsilon; a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; participates in leading-strand synthesis during DNA replication; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p
GO Process (13)
GO Function (5)
GO Component (2)
Gene Ontology Biological Process
- DNA replication proofreading [IMP]
- DNA-dependent DNA replication [IDA]
- base-excision repair [IMP]
- double-strand break repair [IMP]
- double-strand break repair via nonhomologous end joining [IGI, IMP]
- error-prone translesion synthesis [IDA]
- gene conversion [IMP]
- heterochromatin organization involved in chromatin silencing [IGI, IMP]
- intra-S DNA damage checkpoint [IGI, IMP, IPI]
- leading strand elongation [IMP]
- mitotic DNA replication checkpoint [IMP]
- mitotic sister chromatid cohesion [IMP]
- nucleotide-excision repair, DNA gap filling [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
A Network of Conserved Synthetic Lethal Interactions for Exploration of Precision Cancer Therapy.
An emerging therapeutic strategy for cancer is to induce selective lethality in a tumor by exploiting interactions between its driving mutations and specific drug targets. Here we use a multi-species approach to develop a resource of synthetic lethal interactions relevant to cancer therapy. First, we screen in yeast ∼169,000 potential interactions among orthologs of human tumor suppressor genes (TSG) and ... [more]
Mol. Cell Aug. 04, 2016; 63(3);514-25 [Pubmed: 27453043]
Quantitative Score
- -2.73 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- Untreated conditions. SGA was used to score genetic interactions based on the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score >= 2.0 for positive interactions (epistatic or suppressor interactions) and S score <= -2.5 for negative interactions (synthetic sick/lethal interactions).
Curated By
- BioGRID