BAIT
SGS1
ATP-dependent DNA helicase SGS1, L000001877, YMR190C
RecQ family nucleolar DNA helicase; role in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; stimulates DNA catenation/decatenation activity of Top3p; potential repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress
GO Process (18)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
- DNA double-strand break processing [IGI]
- DNA duplex unwinding [IDA]
- DNA topological change [IDA]
- DNA unwinding involved in DNA replication [IDA]
- cellular response to DNA damage stimulus [IMP]
- chromosome organization [IMP]
- double-strand break repair via homologous recombination [IGI, IMP]
- gene conversion at mating-type locus, DNA double-strand break processing [IGI]
- intra-S DNA damage checkpoint [IGI, IMP]
- meiotic DNA double-strand break processing [IGI]
- meiotic chromosome segregation [IMP]
- mitotic sister chromatid segregation [IMP]
- negative regulation of meiotic joint molecule formation [IGI]
- regulation of reciprocal meiotic recombination [IGI]
- replicative cell aging [IMP]
- telomere maintenance [IGI]
- telomere maintenance via recombination [IGI, IMP]
- telomeric 3' overhang formation [IGI]
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
PREY
BET2
L000000172, YPR176C
Beta subunit of Type II geranylgeranyltransferase; required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
GO Process (3)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
A Network of Conserved Synthetic Lethal Interactions for Exploration of Precision Cancer Therapy.
An emerging therapeutic strategy for cancer is to induce selective lethality in a tumor by exploiting interactions between its driving mutations and specific drug targets. Here we use a multi-species approach to develop a resource of synthetic lethal interactions relevant to cancer therapy. First, we screen in yeast ∼169,000 potential interactions among orthologs of human tumor suppressor genes (TSG) and ... [more]
Mol. Cell Aug. 04, 2016; 63(3);514-25 [Pubmed: 27453043]
Quantitative Score
- -2.67 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- Untreated conditions. SGA was used to score genetic interactions based on the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score >= 2.0 for positive interactions (epistatic or suppressor interactions) and S score <= -2.5 for negative interactions (synthetic sick/lethal interactions).
Curated By
- BioGRID