Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.

Rallis C, Townsend S, Baehler J

The Target of Rapamycin (TOR) signalling network plays important roles in aging and disease. The AMP-activated protein kinase (AMPK) and the Gsk3 kinase inhibit TOR during stress. We performed genetic interaction screens using synthetic genetic arrays (SGA) with gsk3 and amk2 as query mutants, the latter encoding the regulatory subunit of AMPK. We identified 69 negative and 82 positive common ... [more]

Sci Rep Mar. 10, 2017; 7();44257 [Pubmed: 28281664]

Quantitative Score

-0.393 [Confidence Score]

Throughput

High Throughput

Ontology Terms

phenotype: colony size (APO:0000063)

Additional Notes

SGA was used to quantitatively scoregenetic interactions based on fitness defects estimated from the colony size of double versus single mutants. Genetic interactions were considered significant if they had a score >= 0.15for positive interactions (epistatic or suppressor interactions)and S score <= -0.15for negative interactions (synthetic sick/lethal interactions).

Curated By

BioGRID

Interaction Summary

GSK3

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein kinase activity [IDA]protein serine/threonine kinase activity [IDA]protein serine/threonine/tyrosine kinase activity [IDA]protein tyrosine kinase activity [IDA]

Gene Ontology Cellular Component

RPL26