BAIT

AAR2

C20orf4, CGI-23
AAR2 splicing factor homolog (S. cerevisiae)
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY

SNRNP40

40K, HPRP8BP, PRP8BP, PRPF8BP, SPF38, WDR57, RP11-490K7.3
small nuclear ribonucleoprotein 40kDa (U5)
UPS Project
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein.

Cloutier P, Poitras C, Durand M, Hekmat O, Fiola-Masson E, Bouchard A, Faubert D, Chabot B, Coulombe B

The R2TP/Prefoldin-like (R2TP/PFDL) complex has emerged as a cochaperone complex involved in the assembly of a number of critical protein complexes including snoRNPs, nuclear RNA polymerases and PIKK-containing complexes. Here we report on the use of multiple target affinity purification coupled to mass spectrometry to identify two additional complexes that interact with R2TP/PFDL: the TSC1-TSC2 complex and the U5 small ... [more]

Nat Commun May. 31, 2017; 8();15615 [Pubmed: 28561026]

Quantitative Score

  • 482.0 [Mascot Score]

Throughput

  • High Throughput

Additional Notes

  • Chromatin
  • False discovery rate (FDR) cutoff was set to 0.1 for the soluble fraction and 0.2 for the chromatin fraction.Some additional known interactors in the soluble fraction with an FDR < 0.2 were included.
  • Soluble

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SNRNP40 AAR2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.7638BioGRID
2271713
SNRNP40 AAR2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9836BioGRID
3147877
AAR2 SNRNP40
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-

Curated By

  • BioGRID