An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein.

Cloutier P, Poitras C, Durand M, Hekmat O, Fiola-Masson E, Bouchard A, Faubert D, Chabot B, Coulombe B

The R2TP/Prefoldin-like (R2TP/PFDL) complex has emerged as a cochaperone complex involved in the assembly of a number of critical protein complexes including snoRNPs, nuclear RNA polymerases and PIKK-containing complexes. Here we report on the use of multiple target affinity purification coupled to mass spectrometry to identify two additional complexes that interact with R2TP/PFDL: the TSC1-TSC2 complex and the U5 small ... [more]

Nat Commun May. 31, 2017; 8();15615 [Pubmed: 28561026]

Quantitative Score

454.0 [Mascot Score]

Throughput

High Throughput

Additional Notes

False discovery rate (FDR) cutoff was set to 0.1 for the soluble fraction and 0.2 for the chromatin fraction.Some additional known interactors in the soluble fraction with an FDR < 0.2 were included.
Soluble

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SFPQ CD2BP2	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Lang B (2021)	High	-	BioGRID	3203802

Curated By

BioGRID

Interaction Summary

CD2BP2

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]ribonucleoprotein complex binding [IDA]

Gene Ontology Cellular Component

SFPQ