BAIT

INO80B

HMGA1L4, HMGIYL4, IES2, PAP-1BP, PAPA-1, ZNHIT4, hIes2
INO80 complex subunit B
GO Process (0)
GO Function (1)
GO Component (5)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

DDX5

G17P1, HLR1, HUMP68, p68
DEAD (Asp-Glu-Ala-Asp) box helicase 5
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein.

Cloutier P, Poitras C, Durand M, Hekmat O, Fiola-Masson E, Bouchard A, Faubert D, Chabot B, Coulombe B

The R2TP/Prefoldin-like (R2TP/PFDL) complex has emerged as a cochaperone complex involved in the assembly of a number of critical protein complexes including snoRNPs, nuclear RNA polymerases and PIKK-containing complexes. Here we report on the use of multiple target affinity purification coupled to mass spectrometry to identify two additional complexes that interact with R2TP/PFDL: the TSC1-TSC2 complex and the U5 small ... [more]

Nat Commun May. 31, 2017; 8();15615 [Pubmed: 28561026]

Quantitative Score

  • 872.0 [Mascot Score]

Throughput

  • High Throughput

Additional Notes

  • False discovery rate (FDR) cutoff was set to 0.1 for the soluble fraction and 0.2 for the chromatin fraction.Some additional known interactors in the soluble fraction with an FDR < 0.2 were included.
  • Soluble

Curated By

  • BioGRID