Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein.
The R2TP/Prefoldin-like (R2TP/PFDL) complex has emerged as a cochaperone complex involved in the assembly of a number of critical protein complexes including snoRNPs, nuclear RNA polymerases and PIKK-containing complexes. Here we report on the use of multiple target affinity purification coupled to mass spectrometry to identify two additional complexes that interact with R2TP/PFDL: the TSC1-TSC2 complex and the U5 small ... [more]
Nat Commun May. 31, 2017; 8();15615 [Pubmed: 28561026]
Quantitative Score
- 250.0 [Mascot Score]
Throughput
- High Throughput
Additional Notes
- False discovery rate (FDR) cutoff was set to 0.1 for the soluble fraction and 0.2 for the chromatin fraction.Some additional known interactors in the soluble fraction with an FDR < 0.2 were included.
- Soluble
Curated By
- BioGRID