BAIT
PDHA1
PDHA, PDHAD, PDHCE1A, PHE1A, RP11-723P2.1
pyruvate dehydrogenase (lipoamide) alpha 1
GO Process (6)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
RPL10A
CSA19, Csa-19, L10A, NEDD6
ribosomal protein L10a
GO Process (14)
GO Function (3)
GO Component (8)
Gene Ontology Biological Process
- RNA metabolic process [TAS]
- SRP-dependent cotranslational protein targeting to membrane [TAS]
- anatomical structure morphogenesis [TAS]
- cellular protein metabolic process [TAS]
- gene expression [TAS]
- mRNA metabolic process [TAS]
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [TAS]
- translation [NAS, TAS]
- translational elongation [TAS]
- translational initiation [TAS]
- translational termination [TAS]
- viral life cycle [TAS]
- viral process [TAS]
- viral transcription [TAS]
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-κB Signaling.
Mitochondrial protein (MP) dysfunction has been linked to neurodegenerative disorders (NDs); however, the discovery of the molecular mechanisms underlying NDs has been impeded by the limited characterization of interactions governing MP function. Here, using mass spectrometry (MS)-based analysis of 210 affinity-purified mitochondrial (mt) fractions isolated from 27 epitope-tagged human ND-linked MPs in HEK293 cells, we report a high-confidence MP network ... [more]
Cell Syst Nov. 07, 2017; (); [Pubmed: 29128334]
Quantitative Score
- 0.000990389 [Confidence Score]
Throughput
- High Throughput
Additional Notes
- HEK293 cell line (Antibody; IP/MS)
- HEK293 cell line (FLAG TAGGED; AP/MS)
- Normalized Spectral Abundance Factor (NSAF) scores were associated with each mitochondrial protein (MP) interaction. High confidence MP interactions were filtered at a confidence threshold of 90%.
Curated By
- BioGRID