VDAC1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PRDX3
Gene Ontology Biological Process
- cellular response to oxidative stress [IDA]
- cellular response to reactive oxygen species [IMP]
- hydrogen peroxide catabolic process [IGI, IMP]
- mitochondrion organization [IMP]
- myeloid cell differentiation [ISS]
- negative regulation of apoptotic process [IDA, IMP]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- negative regulation of kinase activity [IDA]
- peptidyl-cysteine oxidation [IDA]
- positive regulation of NF-kappaB transcription factor activity [IDA]
- positive regulation of cell proliferation [IDA]
- regulation of mitochondrial membrane potential [IMP]
- response to hydrogen peroxide [IDA]
- response to lipopolysaccharide [ISS]
- response to oxidative stress [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-κB Signaling.
Mitochondrial protein (MP) dysfunction has been linked to neurodegenerative disorders (NDs); however, the discovery of the molecular mechanisms underlying NDs has been impeded by the limited characterization of interactions governing MP function. Here, using mass spectrometry (MS)-based analysis of 210 affinity-purified mitochondrial (mt) fractions isolated from 27 epitope-tagged human ND-linked MPs in HEK293 cells, we report a high-confidence MP network ... [more]
Quantitative Score
- 0.00234362 [Confidence Score]
Throughput
- High Throughput
Additional Notes
- HEK293 cell line (FLAG TAGGED; AP/MS)
- Normalized Spectral Abundance Factor (NSAF) scores were associated with each mitochondrial protein (MP) interaction. High confidence MP interactions were filtered at a confidence threshold of 90%.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| VDAC1 PRDX3 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 1.4696 | BioGRID | 2630490 |
Curated By
- BioGRID