BAIT

CLPX

Y75_p0426
ATPase and-specificity subunit of ClpX-ClpP ATP-dependent serine protease
GO Process (0)
GO Function (0)
GO Component (0)
Escherichia coli (K12/W3110)
PREY

UGPE

Y75_p3727
glycerol-3-phosphate transporter subunit
GO Process (0)
GO Function (0)
GO Component (0)
Escherichia coli (K12/W3110)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Global landscape of cell envelope protein complexes in Escherichia coli.

Babu M, Bundalovic-Torma C, Calmettes C, Phanse S, Zhang Q, Jiang Y, Minic Z, Kim S, Mehla J, Gagarinova A, Rodionova I, Kumar A, Guo H, Kagan O, Pogoutse O, Aoki H, Deineko V, Caufield JH, Holtzapple E, Zhang Z, Vastermark A, Pandya Y, Lai CC, El Bakkouri M, Hooda Y, Shah M, Burnside D, Hooshyar M, Vlasblom J, Rajagopala SV, Golshani A, Wuchty S, F Greenblatt J, Saier M, Uetz P, F Moraes T, Parkinson J, Emili A

Bacterial cell envelope protein (CEP) complexes mediate a range of processes, including membrane assembly, antibiotic resistance and metabolic coordination. However, only limited characterization of relevant macromolecules has been reported to date. Here we present a proteomic survey of 1,347 CEPs encompassing 90% inner- and outer-membrane and periplasmic proteins of Escherichia coli. After extraction with non-denaturing detergents, we affinity-purified 785 endogenously ... [more]

Nat. Biotechnol. Nov. 27, 2017; (); [Pubmed: 29176613]

Quantitative Score

  • 7.934622388 [Confidence Score]

Throughput

  • High Throughput

Additional Notes

  • Protein interactions were considered significant if they had a sum of log likelihood scores >= 5.26839785610029, i.e. LLS score >= 5.27.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CLPX UGPE
Positive Genetic
Positive Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

High3.4818BioGRID
1380215

Curated By

  • BioGRID