BAIT

PDCD5

TFAR19
programmed cell death 5
GO Process (1)
GO Function (0)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens
PREY

ATP1B1

ATP1B
ATPase, Na+/K+ transporting, beta 1 polypeptide
GO Process (25)
GO Function (5)
GO Component (6)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

YAF2 promotes TP53-mediated genotoxic stress response via stabilization of PDCD5.

Park SY, Choi HK, Jo SH, Seo J, Han EJ, Choi KC, Jeong JW, Choi Y, Yoon HG

Programmed cell death 5 (PDCD5) plays a crucial role in TP53-mediated apoptosis, but the regulatory mechanism of PDCD5 itself during apoptosis remains obscure. We identified YY1-associated factor 2 (YAF2) as a novel PDCD5-interacting protein in a yeast two-hybrid screen for PDCD5-interacting proteins. We found that YY1-associated factor 2 (YAF2) binds to and increases PDCD5 stability by inhibiting the ubiquitin-dependent proteosomal ... [more]

Biochim. Biophys. Acta May. 01, 2015; 1853(5);1060-72 [Pubmed: 25603536]

Throughput

  • Low Throughput

Curated By

  • BioGRID