Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The small GTPase Rac interacts with ubiquitination complex proteins Cullin-1 and CDC23.

Senadheera D, Haataja L, Groffen J, Heisterkamp N

Racs are involved in the regulation of important cellular processes including mitogenesis. We found that the E3 ubiquitination ligase subunit Cullin-1 interacts with constitutively active Rac3 but not with wild-type Rac3 in yeast. In mammalian cell lysates, Cullin-1 bound to V12Rac3, effector domain mutants V12L37Rac3 and V12H40Rac3, and insert domain deletion mutant V12Rac3DeltaIns(124-135). Cullin-1 also formed a clearly detectable complex ... [more]

Int. J. Mol. Med. Aug. 01, 2001; 8(2);127-33 [Pubmed: 11445862]

Throughput

  • Low Throughput

Curated By

  • BioGRID