Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A Cyclin D2-derived peptide acts on specific cell cycle phases by activating ERK1/2 to cause the death of breast cancer cells.

Russo LC, Araujo CB, Iwai LK, Ferro ES, Forti FL

Protein degradation by the proteasome generates functional intracellular peptides. Pep5, a peptide derived from Cyclin D2, induces cell death in tumor cell lines and reduces the volume of rat C6 glioblastoma tumors in vivo. Here, we chose the human MDA-MB-231 breast cancer cells to evaluate the mechanism of cell death induced by pep5 in different phases of the cell cycle. ... [more]

J Proteomics Jan. 16, 2017; 151();24-32 [Pubmed: 27371349]

Throughput

  • High Throughput

Additional Notes

  • a peptide derived from the bait protein Cyclin D2 (CCND2) interacted with the hit protein as identified by MS in extracts prepared from G1/S synchronized cells

Curated By

  • BioGRID