BAIT
CCND2
KIAK0002, MPPH3
cyclin D2
GO Process (2)
GO Function (2)
GO Component (7)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
HSPA1L
HSP70-1L, HSP70-HOM, HSP70T, hum70t, DADB-333F21.5
heat shock 70kDa protein 1-like
GO Process (2)
GO Function (2)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A Cyclin D2-derived peptide acts on specific cell cycle phases by activating ERK1/2 to cause the death of breast cancer cells.
Protein degradation by the proteasome generates functional intracellular peptides. Pep5, a peptide derived from Cyclin D2, induces cell death in tumor cell lines and reduces the volume of rat C6 glioblastoma tumors in vivo. Here, we chose the human MDA-MB-231 breast cancer cells to evaluate the mechanism of cell death induced by pep5 in different phases of the cell cycle. ... [more]
J Proteomics Jan. 16, 2017; 151();24-32 [Pubmed: 27371349]
Throughput
- High Throughput
Additional Notes
- a peptide derived from the bait protein Cyclin D2 (CCND2) interacted with the hit protein as identified by MS in extracts prepared from S-phase synchronized cells
Curated By
- BioGRID