Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

Mutational analysis of the Arf1*GTP/Arf GAP interface reveals an Arf1 mutant that selectively affects the Arf GAP ASAP1.

Luo R, Jacques K, Ahvazi B, Stauffer S, Premont RT, Randazzo PA

Arf1 is a GTP binding protein that functions at a number of cellular sites to control membrane traffic and actin remodeling. Arf1 is regulated by site-specific GTPase-activating proteins (GAPs). The combined results of crystallographic and biochemical studies have led to the proposal that Arf1 GAPs differ in the specific interface formed with Arf1. To test this hypothesis, we have used ... [more]

Curr. Biol. Dec. 06, 2005; 15(23);2164-9 [Pubmed: 16332543]

Throughput

  • Low Throughput

Additional Notes

  • species of origin for bait and hit is unclear

Curated By

  • BioGRID