BAIT

CEF1

NTC85, L000004270, YMR213W
Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p
Saccharomyces cerevisiae (S288c)
PREY

LSR1

U2, snR20, L000000954
U2 spliceosomal RNA (U2 snRNA), component of the spliceosome; pairs with the branchpoint sequence; functionally equivalent to mammalian U2 snRNA; stress-induced pseudouridylations at positions 56 and 93 may contribute to regulation of splicing
GO Process (2)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae.

Bai R, Yan C, Wan R, Lei J, Shi Y

Removal of an intron from a pre-mRNA by the spliceosome results in the ligation of two exons in the post-catalytic spliceosome (known as the P complex). Here, we present a cryo-EM structure of the P complex from Saccharomyces cerevisiae at an average resolution of 3.6 A. The ligated exon is held in the active site through RNA-RNA contacts. Three bases at the ... [more]

Cell Dec. 14, 2017; 171(7);1589-1598.e8 [Pubmed: 29153833]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CEF1 LSR1
Affinity Capture-RNA
Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Low-BioGRID
-
CEF1 LSR1
Protein-RNA
Protein-RNA

An interaction is detected between and protein and an RNA in vitro.

Low-BioGRID
-

Curated By

  • BioGRID