An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

USP26 functions as a negative regulator of cellular reprogramming by stabilising PRC1 complex components.

Ning B, Zhao W, Qian C, Liu P, Li Q, Li W, Wang RF

Despite much progress in the comprehension of the complex process of somatic cell reprogramming, many questions regarding the molecular mechanism of regulation remain to be answered. At present, the knowledge on the negative regulation of reprogramming process is indeed poor in contrary to the identification of positive regulators. Here we report for the first time that ubiquitin-specific protease 26 negatively ... [more]

Nat Commun Dec. 24, 2016; 8(1);349 [Pubmed: 28839133]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

USP26

Gene Ontology Biological Process

Gene Ontology Molecular Function

cysteine-type endopeptidase activity [IBA]protein binding [IPI]ubiquitin thiolesterase activity [IBA]ubiquitin-specific protease activity [IBA]

Gene Ontology Cellular Component

CBX6